Page 9 - CS03_Hudz

MATERIALS AND METHODOLOGY.

BIOLOGICAL MATERIAL.

 3 bee bread samples were employed in that study.

These samples were removed from the cells of the

hives, packed in polypropylene bags and stored in

refrigeration at (2-8) °C.

 Bee bread granules were put in containers, 50 %

(m/m) ethanol was added in necessary volume

and extraction was carried out at 15-25 ° C with

periodic stirring for 17-21 days at a ratio of raw

material-solvent: 1:10 and 1:5.

 Then extracts were filtered through filter paper. 50

% ethanol was selected as solvent that extracts

both hydrophilic and hydrophobic BAS .