Page 11 - CS03_Hudz

 The amount of 2 % aluminum chloride

hexahydrate in 50 % ethanol was substituted by

the same volume of 50 % etanol in the blank for

each dilution of quercetin dihydrate.

 Simultaneously, each blank for differential

spectrum was used for measuring direct spectrum

of quercetin using 50 % ethanol as blank.

 After incubation, the direct and differential spectra

of the reaction mixtures were measured in range

of 360 nm to 460 nm.